基本信息
貨號：12500 (1mg/1mL)
儲存方式：保持在-20 oC。到期日為自收到之日起一年。
CAS號：61970-00-1
分子量：~60kDa
形式：液體（1mg / mL）
測量范圍：≤1飛摩爾ATP敏感度（使用0.2μg熒光素酶和適當敏感的液體閃爍計數器或發光計）
生物應用
該重組螢火蟲熒光素酶是從來自北美螢火蟲（Photinus pyralis）的克隆基因表達的酶，其提供使用生物發光試劑檢測ATP或熒光素底物進行研究或生產試劑盒所需的可靠性和可靠性。 該重組酶可以潛在地消除可能在從天然來源純化的熒光素酶中發現的季節性和區域變異性的可能性。
該酶與熒光素，ATP和O2的反應導致光的發射。 熒光素酶可用于檢測痕量的ATP。 螢火蟲熒光素酶也是基因表達研究中*常用的報告基因之一。 熒光素酶催化的生物發光反應是測量基因表達的*靈敏的分析工具之一。 通過使用0.2μg熒光素酶可以檢測小于或等于1飛摩爾的ATP。
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