膽堿及其代謝產物在細胞膜的結構完整性和信號傳導以及膽堿能神經傳遞（乙酰膽堿合成）中起著重要作用。它是通過其代謝產物基甘氨酸參與S-腺苷甲硫氨酸合成途徑而獲得甲基的主要來源。膽堿缺乏癥可能導致肝臟病癥，動脈粥樣硬化以及可能的神經系統病癥。盡管膽堿在神經系統中作為乙酰膽堿和膜磷脂酰膽堿的前體具有重要作用，但膽堿在精神病中的作用尚未得到研究。該Amplite 膽堿定量試劑盒提供了靈敏的膽堿定量方法之一。該試劑盒使用Amplite Red量化膽堿的濃度，這與膽堿氧化酶介導的酶偶聯反應中過氧化氫的產生有關。膽堿的量與在酶偶聯反應循環中形成的過氧化氫的濃度成比例。在存在過氧化物酶的情況下，Amplite Red的熒光強度與過氧化氫的形成成比例，該過氧化氫轉化為膽堿的濃度。該測定可以容易地用熒光酶標儀讀取，或者也可以使用光吸收酶標儀在?570 nm處讀取測定值。百螢生物是AAT Bioquest的中國代理商，為您提供的Amplite 膽堿定量試劑盒。

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