Screen Quest 免洗cAMP檢測試劑盒提供了一種便捷的分析方法，用于檢測G蛋白偶聯受體系統中腺苷酸環化酶的活化。與其他商用ELISA cAMP分析試劑盒相比，這種均質的cAMP分析試劑盒不需要洗滌步驟或乙酰化步驟。該測定基于熒光cAMP示蹤劑和未標記的游離cAMP之間固定數目的抗cAMP抗體結合位點的競爭。游離cAMP從HRP-cAMP /抗cAMP抗體復合物中取代了熒光cAMP示蹤劑?？筩AMP抗體用我們的TR Fluor Eu標記，而cAMP示蹤劑包含我們的trFluor 650。在沒有cAMP的情況下，trFluor 650-cAMP綴合物僅與TR Fluor Eu標記的抗cAMP抗體結合。但是，測試樣品中未標記的游離cAMP競爭TR Fluor Eu標記的抗cAMP抗體偶聯物，因此抑制了trFluor 650-cAMP與抗cAMP抗體的結合。 trFluor 650標記的cAMP示蹤劑僅具有納秒級的熒光壽命，而TR Fluor Eu標記的抗cAMP抗體結合的熒光cAMP示蹤劑由于TR-FRET而具有更長的熒光壽命值。 FRET的大小與樣品中cAMP的濃度成正比。該測定可以使用96孔或384孔微孔板進行。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest 免洗cAMP檢測試劑盒。

參考文獻

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