Screen Quest 熒光ELISA cAMP分析試劑盒提供了一種優化的分析方法，用于檢測G蛋白偶聯受體系統中腺苷酸環化酶的。該測定基于HRP標記的cAMP與未標記的cAMP之間固定數目的抗體結合位點的競爭。通過未標記的游離cAMP將HRP-cAMP從HRP-cAMP /抗cAMP抗體復合物中置換出來。在不存在cAMP的情況下，HRP-cAMP偶聯物僅與抗cAMP抗體結合。然而，測試樣品中未標記的游離cAMP與HRP-cAMP抗體偶聯物競爭抗cAMP抗體，因此抑制了HRP-cAMP與抗cAMP抗體的結合。我們的Screen Quest 熒光cAMP分析試劑盒提供了檢測腺苷酸環化酶活性的靈敏方法。與其他商用ELISA cAMP分析試劑盒相比，它繁瑣的乙酰化步驟。該試劑盒使用Amplite Red作為熒光HRP底物來定量HRP活性。形成的熒光產物與HRP-cAMP偶聯物的活性成比例。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest cAMP檢測試劑盒。

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