鈣通量測定法是藥品發現中篩選G蛋白偶聯受體（GPCR）的方法。 Screen Quest Rhod-4 NW鈣含量測定試劑盒提供了一種基于熒光的均相測定，用于檢測細胞內鈣的遷移。Rhod-4是可用于HTS篩選的亮的紅色鈣指示劑。一旦進入細胞內，Rhod-4 的親脂性封閉基團就會被非特異性細胞酯酶裂解，產生帶負電荷的熒光染料，并留在細胞內部，并且與鈣結合后其熒光會大大增強。當細胞被篩選化合物刺激時，受體信號釋放細胞內鈣，這大大增加了Rhod-4的熒光。 Rhod-4 具有長波長，高靈敏度和大于250倍的熒光增強特性（當與鈣形成復合物時），使其成為測量細胞鈣的理想指標。此Screen Quest Rhod-4 NW鈣測定試劑盒提供了一種優化的測定方法，用于檢測G蛋白偶聯受體（GPCR）和鈣通道。該測定可以用96孔或384孔微孔板進行，并易于適應自動化。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest 免洗Rhod-4鈣檢測試劑盒

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