Screen Quest Calbryte 590 免丙磺舒和免洗鈣檢測試劑盒是美國AAT Bioquest研發的用于檢測鈣離子的試劑盒鈣通量測定是用于篩選G蛋白偶聯受體（GPCR）的藥品發現中的優選方法。 Screen Quest Calbryte-590不含Probenecid和Wash-Free的鈣測定試劑盒提供強大的均相熒光測定，用于檢測細胞內鈣動員。表達感興趣的GPCR的細胞通過鈣預先加載我們專有的Calbryte -590NW，其可以穿過細胞膜。 Calbryte -590 NW是適合HTS篩查的鈣指示劑。一旦進入細胞內，Calbryte -590NW的親脂性阻斷基團被非特異性細胞酯酶切割，導致帶電荷的熒光染料停留在細胞內，并且在與鈣結合后其熒光大大增強。當用篩選化合物刺激細胞時，該受體表明細胞內鈣的釋放，這極大地增加了Calbryte -590NW的熒光。其優異的細胞保留特性，高靈敏度和100-250倍的熒光增加（當它與鈣形成復合物時）使Calbryte -590NW成為測量細胞鈣的理想指標.Calbryte -590NW是鈣染料不需要丙磺舒以獲得更好的細胞保留。這款Screen Quest Calbryte-590不含Probenecid和Wash-Free的鈣測定試劑盒提供了優化的檢測方法，用于監測G蛋白偶聯受體（GPCR）和鈣通道與脆弱或困難的細胞系。該測定可以以方便的96孔或384孔微量滴定板形式進行，并且易于適應自動化。百螢生物是AAT Bioquest 的中國代理商，為您提供的鈣檢測試劑盒

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