在進行各種分析的DNA樣品制備中,DNA定量是一項非常重要的任務。 Portelite 熒光DNA定量試劑盒提供了使用Qubit熒光儀操作Helixyte Green BR快速定量dsDNA的方法。它針對Cytocite 和Qubit 熒光儀進行了優化。 Portelite 熒光DNA定量測定在三個數量級上呈線性。該測定法對RNA上的雙鏈DNA(dsDNA)具有高度選擇性,并針對測量10 pg / μL至10 ng / μL的dsDNA濃度進行了優化。Helixyte Green-BR與dsDNA結合后表現出較大的熒光增強,并且比UV吸光度讀數高幾個數量級。百螢生物是AAT Bioquest的中國代理商,為您提供的Helixyte 綠色雙鏈DNA定量檢測試劑盒。
參考文獻
A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads
Authors: Choi, Y., Schmidt, C., Tinnefeld, P., Bald, I., Rodiger, S.
Journal: Sci Rep (2019): 4769
A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
Authors: Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.
Journal: Sci Rep (2019): 8853
Effects of Quantification Methods, Isolation Kits, Plasma Biobanking, and Hemolysis on Cell-Free DNA Analysis in Plasma
Authors: Streleckiene, G., Forster, M., Inciuraite, R., Lukosevicius, R., Skieceviciene, J.
Journal: Biopreserv Biobank (2019): ersion="1.0" encoding="UTF-8" ?>17645.enlEndNote1117Streleckiene, G.Forster, M.Inciuraite, R.Lukosevicius, R.Skieceviciene, J.1Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 2Institute of Clinical Molecu