檢測和定量少量 RNA 對于各種生物學應用極為重要,例如在進行 Northern 印跡分析、S1 核酸酶測定、RNase 保護測定、cDNA 文庫制備、逆轉錄之前測量體外轉錄 RNA 的產量和測量 RNA 濃度PCR、差異顯示PCR。測量核酸濃度常用的技術是測定 260 nm 處的吸光度?;谖舛鹊姆椒ㄊ艿降鞍踪|、游離核苷酸和其他紫外線吸收化合物引起的干擾的限制。使用敏感且選擇性的熒光核酸染色劑可以緩解這種干擾問題。 StrandBrite? RNA 定量試劑是一種超靈敏熒光核酸染色劑,用于定量溶液中的 RNA。 StrandBrite? RNA 定量試劑可使用 CytoCite? 或 Qubit? 熒光計檢測低至 5 ng/mL 的 RNA。我們的 StrandBrite? Green 熒光 RNA 定量試劑盒包括我們的 StrandBrite? Green 核酸染色劑和優化且穩定的實驗方案。它提供了一種定量溶液中 RNA 的便捷方法。。
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